Physical organization of mixed protease inhibitor gene clusters, coordinated expression and association with resistance to late blight at the StKI locus on potato chromosome III.
Identifieur interne : 001863 ( Main/Exploration ); précédent : 001862; suivant : 001864Physical organization of mixed protease inhibitor gene clusters, coordinated expression and association with resistance to late blight at the StKI locus on potato chromosome III.
Auteurs : Damaris Achieng Odeny [Allemagne] ; Benjamin Stich ; Christiane GebhardtSource :
- Plant, cell & environment [ 1365-3040 ] ; 2010.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Analyse de séquence d'ADN (MeSH), Cartographie physique de chromosome (MeSH), Chromosomes artificiels de bactérie (MeSH), Chromosomes de plante (MeSH), Expression des gènes (MeSH), Famille multigénique (MeSH), Interactions hôte-pathogène (MeSH), Locus de caractère quantitatif (MeSH), Maladies des plantes (génétique), Peptides (génétique), Phytophthora infestans (physiologie), Protéines végétales (génétique), Solanum tuberosum (génétique), Solanum tuberosum (immunologie), Solanum tuberosum (microbiologie).
- MESH :
- génétique : Maladies des plantes, Peptides, Protéines végétales, Solanum tuberosum.
- immunologie : Solanum tuberosum.
- microbiologie : Solanum tuberosum.
- physiologie : Phytophthora infestans.
- Amorces ADN, Analyse de séquence d'ADN, Cartographie physique de chromosome, Chromosomes artificiels de bactérie, Chromosomes de plante, Expression des gènes, Famille multigénique, Interactions hôte-pathogène, Locus de caractère quantitatif.
English descriptors
- KwdEn :
- Chromosomes, Artificial, Bacterial (MeSH), Chromosomes, Plant (MeSH), DNA Primers (MeSH), Gene Expression (MeSH), Host-Pathogen Interactions (MeSH), Multigene Family (MeSH), Peptides (genetics), Physical Chromosome Mapping (MeSH), Phytophthora infestans (physiology), Plant Diseases (genetics), Plant Proteins (genetics), Quantitative Trait Loci (MeSH), Sequence Analysis, DNA (MeSH), Solanum tuberosum (genetics), Solanum tuberosum (immunology), Solanum tuberosum (microbiology).
- MESH :
- chemical , genetics : Peptides, Plant Proteins.
- chemical : DNA Primers.
- genetics : Plant Diseases, Solanum tuberosum.
- immunology : Solanum tuberosum.
- microbiology : Solanum tuberosum.
- physiology : Phytophthora infestans.
- Chromosomes, Artificial, Bacterial, Chromosomes, Plant, Gene Expression, Host-Pathogen Interactions, Multigene Family, Physical Chromosome Mapping, Quantitative Trait Loci, Sequence Analysis, DNA.
Abstract
Protease inhibitors (PIs) play a role in plant defence against pests and pathogens as well as in plant development. Potato (Solanum tuberosum) contains abundant levels of diverse PIs. Most potato Kunitz-type inhibitor (KTI) genes map to the StKI locus on potato chromosome III, which is linked to a quantitative trait locus (QTL) for resistance to Phytophthora infestans. To elucidate the physical organization of PIs at the StKI locus, we screened bacterial artificial chromosome (BAC) libraries with KTI probes. Ten different clones were selected, sequenced and annotated. Of 100 putative genes, 22 corresponded to five PI classes. Expression analysis by quantitative real-time PCR (qRT-PCR) using PI class-specific primers in different tissues of the tetraploid potato cultivars 'Nikita' and 'Baltica' revealed different transcript levels, depending on PI type and genotype. During the compatible interaction with a complex race of P. infestans, four PI classes showed coordinated expression over 3 d after infection, a strong decrease in infected leaves and a transient induction in systemic leaves. Basal transcript levels in non-infected leaves differed strongly between the two genotypes examined. Two microsatellite markers located within the PI gene cluster were associated with resistance to P. infestans in a population of potato varieties and breeding clones.
DOI: 10.1111/j.1365-3040.2010.02213.x
PubMed: 20716067
Affiliations:
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at the StKI locus on potato chromosome III.</title><author><name sortKey="Odeny, Damaris Achieng" sort="Odeny, Damaris Achieng" uniqKey="Odeny D" first="Damaris Achieng" last="Odeny">Damaris Achieng Odeny</name><affiliation wicri:level="3"><nlm:affiliation>Max-Planck Institute for Plant Breeding Research, Köln, Germany. dodeny@arc.agric.za</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max-Planck Institute for Plant Breeding Research, Köln</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Stich, Benjamin" sort="Stich, Benjamin" uniqKey="Stich B" first="Benjamin" last="Stich">Benjamin Stich</name></author><author><name sortKey="Gebhardt, Christiane" sort="Gebhardt, Christiane" uniqKey="Gebhardt C" first="Christiane" last="Gebhardt">Christiane 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Bacterial</term><term>Chromosomes, Plant</term><term>Gene Expression</term><term>Host-Pathogen Interactions</term><term>Multigene Family</term><term>Physical Chromosome Mapping</term><term>Quantitative Trait Loci</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Analyse de séquence d'ADN</term><term>Cartographie physique de chromosome</term><term>Chromosomes artificiels de bactérie</term><term>Chromosomes de plante</term><term>Expression des gènes</term><term>Famille multigénique</term><term>Interactions hôte-pathogène</term><term>Locus de caractère quantitatif</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Protease inhibitors (PIs) play a role in plant defence against pests and pathogens as well as in plant development. Potato (Solanum tuberosum) contains abundant levels of diverse PIs. Most potato Kunitz-type inhibitor (KTI) genes map to the StKI locus on potato chromosome III, which is linked to a quantitative trait locus (QTL) for resistance to Phytophthora infestans. To elucidate the physical organization of PIs at the StKI locus, we screened bacterial artificial chromosome (BAC) libraries with KTI probes. Ten different clones were selected, sequenced and annotated. Of 100 putative genes, 22 corresponded to five PI classes. Expression analysis by quantitative real-time PCR (qRT-PCR) using PI class-specific primers in different tissues of the tetraploid potato cultivars 'Nikita' and 'Baltica' revealed different transcript levels, depending on PI type and genotype. During the compatible interaction with a complex race of P. infestans, four PI classes showed coordinated expression over 3 d after infection, a strong decrease in infected leaves and a transient induction in systemic leaves. Basal transcript levels in non-infected leaves differed strongly between the two genotypes examined. Two microsatellite markers located within the PI gene cluster were associated with resistance to P. infestans in a population of potato varieties and breeding clones.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20716067</PMID><DateCompleted><Year>2011</Year><Month>03</Month><Day>10</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>12</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ</ISOAbbreviation></Journal><ArticleTitle>Physical organization of mixed protease inhibitor gene clusters, coordinated expression and association with resistance to late blight at the StKI locus on potato chromosome III.</ArticleTitle><Pagination><MedlinePgn>2149-61</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-3040.2010.02213.x</ELocationID><Abstract><AbstractText>Protease inhibitors (PIs) play a role in plant defence against pests and pathogens as well as in plant development. Potato (Solanum tuberosum) contains abundant levels of diverse PIs. Most potato Kunitz-type inhibitor (KTI) genes map to the StKI locus on potato chromosome III, which is linked to a quantitative trait locus (QTL) for resistance to Phytophthora infestans. To elucidate the physical organization of PIs at the StKI locus, we screened bacterial artificial chromosome (BAC) libraries with KTI probes. Ten different clones were selected, sequenced and annotated. Of 100 putative genes, 22 corresponded to five PI classes. Expression analysis by quantitative real-time PCR (qRT-PCR) using PI class-specific primers in different tissues of the tetraploid potato cultivars 'Nikita' and 'Baltica' revealed different transcript levels, depending on PI type and genotype. During the compatible interaction with a complex race of P. infestans, four PI classes showed coordinated expression over 3 d after infection, a strong decrease in infected leaves and a transient induction in systemic leaves. Basal transcript levels in non-infected leaves differed strongly between the two genotypes examined. Two microsatellite markers located within the PI gene cluster were associated with resistance to P. infestans in a population of potato varieties and breeding clones.</AbstractText><CopyrightInformation>© 2010 Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Odeny</LastName><ForeName>Damaris Achieng</ForeName><Initials>DA</Initials><AffiliationInfo><Affiliation>Max-Planck Institute for Plant Breeding Research, Köln, Germany. dodeny@arc.agric.za</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stich</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Gebhardt</LastName><ForeName>Christiane</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>09</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C053865">Kunitz-type protease inhibitor, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D022202" MajorTopicYN="N">Chromosomes, Artificial, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032461" MajorTopicYN="Y">Chromosomes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="Y">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="N">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020161" MajorTopicYN="N">Physical Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040641" MajorTopicYN="N">Quantitative Trait Loci</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>3</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20716067</ArticleId><ArticleId IdType="pii">PCE2213</ArticleId><ArticleId IdType="doi">10.1111/j.1365-3040.2010.02213.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>District de Cologne</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Cologne</li></settlement></list><tree><noCountry><name sortKey="Gebhardt, Christiane" sort="Gebhardt, Christiane" uniqKey="Gebhardt C" first="Christiane" last="Gebhardt">Christiane Gebhardt</name><name sortKey="Stich, Benjamin" sort="Stich, Benjamin" uniqKey="Stich B" first="Benjamin" last="Stich">Benjamin Stich</name></noCountry><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Odeny, Damaris Achieng" sort="Odeny, Damaris Achieng" uniqKey="Odeny D" first="Damaris Achieng" last="Odeny">Damaris Achieng Odeny</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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